Fig. 7From: Plumbagin can potently enhance the activity of xanthine oxidase: in vitro, in vivo and in silico studiesKinetic measurement of the binding affinity of PLB to XO by BLI assay. Kinetic binding sensorgrams of increasing concentrations of PLB from 25 to 200 μM were shown with real-time data acquisition for each step of the kinetic assay. Response/binding (nm) represents the optical thickness of the sensor layer reflected by the spectral shift (1λ) upon the interaction of PLB with XO. The response at steady state where the rate of association is equal to the dissociation and the equilibrium binding signal (Req) indicated by the flattened curve were reachedBack to article page