Fig. 4
The inability of VGB to alter the activity of BKCa channels was recorded in human 13–06-MG cells. The experiments were conducted under cell-attached configuration. The cells were bathed in a high-K+ solution containing 1.8 mM CaCl2, and the examined cells were clamped at a level of + 80 mV. a Original trace of single BKCa channels obtained in the control (upper) and after the addition of 10 μM VGB (middle) or 10 μM VGB plus 10 μM GAL-021 (lower). The upward deflection indicates the opening event of the channel. b Summary bar graph of the effects of VGB or VGB plus GAL-021 on the probability of BKCa channel opening (mean ± SEM; n=7 for each bar). *Significantly different from the control or 10 μM VGB alone (P< 0.05)