Fig. 3

Effect of 100 μM NAC on the ROS increase and GSH decrease induced by 50 μg/mL BPA.C, control; NAC, 100 μM N-acetyl-L-cysteine; BPA, 50 μg/mL bisphenol A; NAC + BPA, combined treatment with N-acetyl-L-cysteine (100 μM) and bisphenol A (50 μg/mL). After 14 h of maturation, (a) ROS levels (average fluorescence intensity value) in the control, NAC, BPA and NAC + BPA groups were 7.1 ± 0.2, 4.1 ± 0.4, 22.6 ± 1.2 and 7.9 ± 0.8, respectively. ROS levels significantly decreased in the combination group compared with the BPA group. Each treatment was repeated 3–4 times, with each replicate including approximately 10 oocytes. (b) GSH content significantly increased in the combination group compared with the BPA group. Each treatment was repeated 3 times, with each replicate including approximately 25 oocytes.^a-cValues with a different letter above the bars differ significantly (P < 0.05)