Table 5 Biochemical properties of some microbial L-asparaginases
Microbial source | pH optima | Temperature optima (°C) | Km (M) | Vmax | Specific activity (U/mg protein) | Molecular weight (kDa) | References |
|---|---|---|---|---|---|---|---|
Streptomyces albidoflavus | 7.5 | 35 | – | – | 437 | 112 | [29] |
Streptomyces gulbargensis | 9 | 40 | – | – | 2053 | 85 | [13] |
Streptomyces radiopugnans MS | 6 | 40 | 5.98 × 10−2 | 3.547 IU/μg | 5035.28 | 133.25 | [36] |
Enterobacter cloacae | 7–8 | 35–40 | 1.58 × 10−3 | 2.22 IU/μg | 105.07 | 52 | [37] |
Pseudomonas aeruginosa | 9 | 37 | 0.147 × 10− 3 | 35.7 IU | 1900 | 160 | [30] |
Vibrio cholerae | 7 | 37 | 1.1 × 10− 3 | 1006 μM/min | 648.9 | 132 | [21] |
Pseudomonas stutzeri | 9 | 37 | 1.45 × 10−4 | – | 732.3 | 34 | [38] |
Pseudomonas fluorescens | 8–9 |  | 4.1 × 10− 4 | – | 500 | 70 | [39] |
Azotobacter vinelandii | 8.6 | 48 | 1.1 × 10− 4 |  | 2.47 | 84 | [40] |
E. coli | 7–8 | 37 | 3.5 × 10− 3 | – | 150–250 | 139 | |
Erwinia aroideae | 8.2 | 45 | 2.8 × 10− 3 | – | – | 155 | [44] |
Corynebacterium glutamicum | 7 | 40 | 2.5 × 10− 3 | – | 2020 | 80 | [45] |
Bacillus coagulans | 8.8–9.7 | 55 | 5 × 10− 3 | 5.83 × 10− 5 M/h | 10.9 | 84 | [46] |
Bacillus licheniformis | 6–10 | 40 | 1.4 × 10−5 | 4.03 IU | 697.09 | 134.8 | [47] |
Aspergillus oryzae CCT 3940 | 8 | 50 | 0.66 × 10−3 | 313 IU/mL | 91 | 115 | [48] |
Fusarium culmorum ASP-87 | 8 | 45 | 3.57 × 10−3 | 0.5 μmol/mL/min | 16.66 | 90 | [22] |