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Table 1 Summary of the purification steps of the L-asparaginase produced by Streptomyces brollosae NEAE-115

From: Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Purification step

Total protein content (mg)

L-asparaginase activity

Recovery (%)

Purification fold

Total activity (U)

Specific activity (U/mg protein)

Culture filtrate

3120.42

30538.87

9.786

100

1

(NH4)2SO4, post dialysis

158.86

10,962

69.004

35.895

7.051

Ion exchange on DEAE Sepharose

10.371

795.152

76.671

7.254

7.835