Fig. 14
(Abstract A70). Effects of BAY60-2770 on TNF-induced leukocyte adhesion and recruitment in SCD mice. Effects of BAY60-2770 on TNF-induced leukocyte adhesion and recruitment in SCD. a Neutrophils from steady-state SCA patients (N=7) were incubated, or not, with BAY 60-2770 (0.1 – 100 μM; 120 min) before stimulating with TNF (200 ng/ml) and allowed to adhere to fibronectin-coated plates (30 min, 37oC, 5% CO2). ***, P < 0.001 compared to basal; #. P<0.05, ##P<0.01, compared to TNF. b-d Intravital microscopy of the cremaster microcirculation of chimeric SCD mice: (b) Leukocyte adhesion and (c) leukocyte extravasation at 180 min after TNF-α administration (TNF, 0.5 μg i.p.) and with co-administration of hydroxyurea (HU, 100 μg/kg) and/or BAY60-2770 (BAY 60, 10 μg/mouse). N = 32-39 venules of 3-4 mice per group; ***, P<0.05, compared to CON mice that also received TNF; ###, P<0.05, compared to TNF. Bar= 20μm. Mann-Whitney or AVOVA/Sidak’s test, as appropriate