Fig. 2

The proteins expression of autophagy in TNF-α-treated HCAECs in the presence of RSV. HCAECs were either untreated (CON) or treated by TNF-α 10 ng/ml for 2 h in the presence or absence of RSV 10 μM (RSV). The Western blots illustrate the expression of Atg16L1, Beclin 1 and LC3B proteins in cytosolic extracts of HCAECs. The results shown are representative of three separate experiments. GAPDH worked as a normalization of cytosolic protein. Representative immunoblots (a) and densitometric quantification of immunoreactive bands are shown. The relative band intensities of Atg16L1 (b), Beclin 1 (c) and LC3-II (d) in HCAECs were quantified as fold increases compared with the control cells. Each value represents the mean ± S.E.M. of 3 independent experiments. An asterisk indicates a significant difference (p < 0.05)