Figure 1From: Mutation of a conserved lysine in the kinase homology domain reduces the natriuretic peptide-dependent activity and phosphorylation of guanylyl cyclase-AProQ Diamond staining of phosphate concentrations and Coomassie staining of protein concentrations of wild type GC-A and K535A-GC-A purified from transiently transfected 293T cells by sequential immunoprecipitation and SDS-PAGE fractionation. Green fluorescent protein (GFP) is a negative control for the immunoprecipitation of GC-A.Back to article page