Figure 4

Effect of CNX-010-49 on adipocytes differentiation, hypertrophy and lipolysis. Mouse 3T3-L1 preadipocytes were differentiated into mature adipocytes using complete differentiating media (CDM) in the presence and absence of 1 μM of CNX-010-49. The extent of inhibition of adipocytes differentiation was assessed by measuring triglyceride levels (A) and Oil red O staining (B). For inhibition of lipolysis, mature adipocytes were treated with 1 μM isoproterenol and 100 nM cortisone with or without 1 μM of CNX-010-49 for 18 h. Post 18 h, glycerol released into the media was measured (C). For inhibition of adipose hypertrophy, mature adipocytes were treated with GPCI (25 mM glucose, 500 μM palmitate, 1 μM cortisone, 10 ng/ml of inflammatory cytokines (TNFa, IL1 β, IFNγ)) for 48 h with or without 1 μM of CNX-010-49. Post 48 h, triglyceride accumulation was measured (D). All the values are expressed as Mean ± SEM. Statistical comparison was conducted by One-way ANOVA followed by Dunnett’s test (n = 6) (*P < 0.05, **P < 0.01 and ***P < 0.001).