Figure 4From: Conditional disruption of interactions between Gαi2 and regulator of G protein signaling (RGS) proteins protects the heart from ischemic injury Gα i2 protein expression is reduced in mice conditionally expressing Gα i2 G184S . Gαi2 protein expression was measured by Western blot in hearts (A), brains (B), and kidneys (C) isolated from mice treated with tamoxifen (50 mg/kg; shaded bars) or vehicle (nonshaded bars) for 5 consecutive days. The membrane was stripped and reblotted with a GAPDH antibody as a loading control. Values represent the mean ± SE of tissues from three different animals for each genotype. Data are normalized to Gαi2 protein expression in tissues from wildtype C57B/6J mice that do not express Cre and do not carry the Gαi2 G184S mutation. Panel D shows a representative blot of kidneys from three different animals of each genotype. * and ** indicate significant differences (p < 0.05 and p < 0.01, respectively) relative to control mice (Gαi2 +/+) that do not carry the Cre-ERT2 or Gαi2 fl-G184S/fl-G184S genes.Back to article page