Table 2 The estimated LD 50 of Yessotoxin (YTX) in different developmental stages of RSP design
From: The development of response surface pathway design to reduce animal numbers in toxicity studies
Design | Dose (μg/kg BW) | Proportion of dead mice | LD50with 95% CI (μg/kg BW) | |
|---|---|---|---|---|
Isotonic regression | Spearman-Karber | |||
Basic RSP | 400 | 3/9 (0.33) | 463 (383 – 535) | 457 (400 – 522) |
450 | 4/9 (0.44) | |||
500 | 7/9 (0.78) | |||
600 | 5/9 (0.56) | |||
*) Included k-adjustment factor | 400 | 3/9 (0.33) | 481 (362 – 566) | 480 (408 – 565) |
462 | 4/9 (0.44) | |||
499 | 5/9 (0.56) | |||
581 | 5/9 (0.56) | |||
*) Optimising use of mice | 400 | 1/3 (0.33) | 447 (378 – 504) | 444 (379 – 521) |
462 | 5/9 (0.56) | |||
499 | 5/7 (0.71) | |||
581 | 4/5 (0.80) | |||
*) Multinomial decision variable with all four design levels | 400 | 1/3 (0.33) | 473 (442 – 517) | 471 (430 – 516) |
465 | 3/7 (0.43) | |||
468 | 4/9 (0.44) | |||
482 | 3/5 (0.60) | |||